@article{MAKHILLJAVA201211103437,
    title = {A Simple Cultural Method for Detection of <I>Mycoplasma bovis</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {10},
    pages = {1643-1646},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.1643.1646},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.1643.1646},
    author = {Nining,Qian,Zhang and},
    keywords = {Culture,detect,method,Mycoplasma bovis,media,CO2},
    abstract = {Simple method for the detection of <I>Mycoplasma bovis</I> in both liquid and solid cultures was established. Fresh media were easily prepared and dispatched into an eppendorf tube or a Petri dish, a new cultural procedure was followed with the eppendorf tube closed or the Petri dish sealed. Compared to the conventional methods, appropriate inoculating and subculturing operations got easier and a CO<SUB>2</SUB> incubator was not required; moreover, probable contamination by airborne fungi or bacteria could be reduced or eliminated during long-term incubation. Similarly, <I>M. bovis</I> grew normally and could be investigated by Color-Change Units (CCU) enumeration, colony morphology or PCR assay.}
    }