@article{MAKHILLJAVA20121193415,
    title = {Immunogenicity of Foot and Mouth Disease Virus Type Asia 1 Protein VP1-2A Fused with a Multi-Epitope Expressed in <I>Pichia pastoris</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {9},
    pages = {1512-1517},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.1512.1517},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.1512.1517},
    author = {Ningyi Jin,Zhan-Bo,Ying Xi,Bo Hu,Chang Li,Huijun Lu,Kuoshi Jin,Jingqiang Ren,Cunxia Liu and},
    keywords = {FMDV,VP1-2A,CTE,Pichia pastoris,immunogenicity,China},
    abstract = {Foot and Mouth Disease (FMD) is a highly infectious and economically devastating disease of livestock. A genetically engineered vaccine against Foot and Mouth Disease Virus (FMDV) may be a good control measure for use during an epidemic. In this study, a secretory expression vector, pPIC9K-VP1-2A-CTE, containing a fusion of the bovine (FMDV) type Asia 1 <I>VP1-2A </I>gene and a multi-epitope CTB-TEpi (CTE) was constructed. After being linearized with Sal I, the recombinant plasmid was transformed into <I>Pichia pastoris</I> GS115 by electroporation. The fusion protein expressed in yeast was analyzed by SDS-PAGE and Western blot and immunogenicity of the fusion protein was assessed. The expressed recombinant VP1-2A and CTE protein elicited specific humoral and cellular immune responses in mice. Additionally, immunized guinea pigs were protected when challenged with the FMD Asia 1 virus.}
    }