@article{MAKHILLJAVA20121183365,
    title = {Construction of RNAi Expressing Vector Targeting the Goat Prion Protein Gene (<I>PRNP</I>)},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {8},
    pages = {1208-1212},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.1208.1212},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.1208.1212},
    author = {Yongjun,Wenting Li,Taikun Li,Guangjing Zhang,Jiahui Zhang and},
    keywords = {RNA interference,siRNA,prion protein gene,goat,antagonistic,protein},
    abstract = {To explore the effect of RNA interference on the suppression of goat prion protein gene (<I>PRNP</I>), small interfering RNA (siRNA) expressing vectors pSilencer4.1-siRNA1 and a negative control pSilencer4.1-siRNA2 and the eukaryotic expressing vector pSG5-prion expressing parts of goat prion protein gene were designed and constructed. The recombinants were introduced into Human Embryonic Kidney (HEK) 293T cells mediated by GeneTran. RT-PCR was carried out to detect the expression of prion protein gene. The results showed that RNAi effects induced by pSilencer4.1-siRNA1 could successfully deplete the mRNA expression of goat prion protein gene in 293T cells 48 h after transfection with both vectors compared to the negative control. The findings demonstrated that pSilencer4.1-siRNA1 was constructed successfully to transfer antagonistic substance against goat prion protein gene.}
    }