@article{MAKHILLJAVA20121183358,
    title = {Establishment of Two Types of Mammary Epithelial Cell Lines from Chinese Holstein Dairy Cow},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {8},
    pages = {1166-1172},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.1166.1172},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.1166.1172},
    author = {Zhihui Zhao,Chunyan,Runjun Yang,Boyang Liu,Zezhong Li,Binglei Shen,Shouqing Yan,Yonghong Zhang and},
    keywords = {Mammary epithelial,Chinese Holstein dairy cow,cell lines,fat,protein,China},
    abstract = {Chinese Holstein dairy cow is the main dairy cow in China. 
  With the development of Chinese milk industry, more attention is being placed 
  on the factors that might affect milk quality, especially milk fat and milk 
  protein content. So, the objective of this study was to establish a culture 
  system for two types of bovine mammary epithelial cell line, high fat high protein 
  Mammary Epithelial Cell line (MEC-HH) and low fat low protein Mammary Epithelial 
  Cell Line (MEC-LL) <em>in vitro</em> and elucidate the unique characteristics 
  of these cell lines. Mammary tissues from two lactating dairy cows whose milk 
  were high fat high protein content and low fat low protein content were used 
  as sources of these epithelial cell lines. The tissues were cultured in plastic 
  dishes by the method of tissue nubbles culture. Fibroblast and epithelial cells 
  successively grew and extended from mammary tissues after 6 days from low fat 
  low protein cow but 10 days from high fat high protein cow and pure epithelial 
  cells were obtained by continuous culture and digestion for 3-5 times. The epithelial 
  cells cultured in the 10% FBS maintained a normal growth tendency and had normal 
  cell nucleus. The &alpha;-s1 casein and &kappa;-casein expression of epithelial 
  cells were detected by RT-PCR. The positive immunocytochemistry to cytokeratin 
  18 suggested the obtained cell line exhibited the specific character of epithelial 
  cells. A TG-kit was used to detect the TG content of the two types of cells, 
  the results showed that the cell lines kept the character of high fat and low 
  fat cows in the 1st and 2nd passages. The obtained mammary epithelial cell lines 
  had normal morphology, growth characteristics, cytogenetic and secretory characteristics. 
  Thus, it might supply a useful tool for studying the gene function about milk 
  fat and protein. The research provides the basis for the future study of lipometabolism 
  mechanism in dairy cow.}
    }