@article{MAKHILLJAVA20121183351,
    title = {Cloning and Expression of Transforming Growth Factor Beta 1 of Guanzhong Dairy Goat and its Effect on Proliferation of Goat Peripheral Blood Mononuclear Cells (PBMCs)},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {11},
    number = {8},
    pages = {1127-1134},
    year = {2012},
    issn = {1680-5593},
    doi = {javaa.2012.1127.1134},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2012.1127.1134},
    author = {D.K. Chen,C.C.,Y.Q. Zhao,D.Y. Cao,Y.L. Yao and},
    keywords = {TGF-B1,PBMCs,proliferation,dairy goat,physiological state,amino acid},
    abstract = {Transforming Growth Factor beta 1 (TGF-&#946;1) is a pleiotropic cytokine that affects diverse biologic processes. In this study, the goat <I>TGF-&#946;1</I> gene was cloned and sequenced with length of 1173 bp. The predicted peptide of goat TGF-&#946;1 contained 390 amino acids and 29 amino acids signal peptide. Phylogenetic analysis of different species available in GenBank<SUP>TM</SUP> based on the complete amino acid sequences of TGF-&#946;1 showed that the goat was clustered with sheep sistered to bovine. The TGF-&#946;1 mature peptide including 112 amino acids was expressed with a fusion protein of approximately 32 kDa. The goat PBMCs were proliferative stimulated by recombinant TGF-&#946;1 but the proliferation of PBMCs stimulated by ConA was inhibited under co-stimulation of recombinant TGF-&#946;1. These results suggest that the goat recombinant TGF-&#946;1 would play an important role in regulating the immune responses and sustaining the balance of organism physiological state.}
    }