@article{MAKHILLJAVA20111082702,
    title = {Populations of <I>Salmonella enteritidis</I> in the Tissues of Reproductive Organs in Laying Hens After Oral Challenge: A Real-Time PCR Study},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {8},
    pages = {945-948},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.945.948},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.945.948},
    author = {Guang-Zhi,Wei-Yi,Ning and},
    keywords = {Salmonella enteritidis,reproductive organs,population,vagina,concentration,China},
    abstract = {The objective of this study was to determine the populations of <I> Salmonella enteritidis</I> (<I>S. enteritidis)</I> in reproductive organs of laying hens after oral challenge. Researchers conducted serovar-specific Real-time PCR for <I>S. enteritidis</I> to detect the genomic DNA of <I>S. enteritidis</I> from laying hens at different time points. To validate these results, the Indirect Fluorescent Antibody (IFA) technique was employed too. The results showed that <I>S. enteritidis</I> was consistently detected in all the samples. Vagina and uterus were positive at 20 h PI and the last organ to show a positive result was the largest and third largest preovulatory follicle at 32 h PI. The copy numbers of <I>S. enteritidis</I> DNA in each tissue reached a peak at 36-60 h PI with the vagina and uterus containing higher concentrations than other tissues. However, the number of bacteria started decreasing by 3-4 days and by 6 days, the concentration of <I>S. enteritidis</I> DNA was below the detection limits of the PCR assay except the vagina. In conclusion, the results provided insights into the <I>S. enteritidis</I> populations in the reproductive organs. This study will help in understanding the pathogenesis of <I>S. enteritidis</I> infection <I>in vivo</I>.}
    }