@article{MAKHILLJAVA20111012549,
    title = {Development and Validation of a Gas Chromatography-Mass Spectrometry Method for the Simultaneous Determination of Melamine and Cyromazine in Animal Feeds},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {1},
    pages = {73-80},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.73.80},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.73.80},
    author = {Liying,Wenjun,Zongyi,Yiqiang and},
    keywords = {determination,cyromazine,gas chromatography-mass spectrometry,feeds,Melamine},
    abstract = {A new method for simultaneous determination of melamine and cyromazine in animal feeds using Gas Chromatography-Mass Spectrometry (GC-MS) was developed and validated. Samples were extracted with trichloroacetic acid solution cleaned up by cation exchange solid-phase extraction cartridges and derivatized with N, O-bis (trimethylsilyl) trifluroacetamide containing 1% trimethylchlorosilane followed by GC separation and MS detection. The limits of quantification were 0.10 mg kg<SUP>-1</SUP> for both melamine and cyromazine. Recoveries from feeds spiked at levels between 0.1 and 50 mg kg<SUP>-1</SUP> ranged from 84.2-99.5% with Relative Standard Deviation (RSD) &lt;8% with the exception of a 10.2% RSD for 0.1 mg kg<SUP>-1</SUP> melamine. This validated method was successfully applied to commercial feed samples showing that it can be used as a routine tool for the surveillance and evaluation of the presence of melamine and cyromazine in animal feeds.}
    }