@article{MAKHILLJAVA20111052633,
    title = {Characterisation of <I>Angiostrongylus cantonensis</I> Isolates from China by Sequences of Internal Transcribed Spacers of Nuclear Ribosomal DNA},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {5},
    pages = {593-596},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.593.596},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.593.596},
    author = {R.Q.,X.Q.,C.Y.,M.X.,L.,J.,R.L. and},
    keywords = {China,sequence analysis,Polymerase Chain Reaction (PCR),Angiostrongylus cantonensis,Internal Transcribed Spacer (ITS),ribosomal DNA (rDNA)},
    abstract = {The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) among <I>Angiostrongylus cantonensis </I>isolates from Shenzhen, Qingyuan, Jiangmen and Wenzhou in China. The ITS of nuclear rDNA was amplified from individual <I>A. cantonensis </I>by Polymerase Chain Reaction (PCR) and the representative amplicons were cloned and sequenced. The length of the ITS sequences was 1593-1614 bp for all Chinese <I>A. cantonensis</I> specimens and these sequences were composed of complete ITS-1 sequence of 712-720 bp, complete 5.8 S sequence of 153 bp, complete ITS-2 sequence of 633-650 bp and partial 28 S sequence of 70 bp. The intra-specifc sequence variation in <I>A. cantonensis</I> was 0.1-1.0% for ITS-1 and 0.0-1.3% for ITS-2 whereas sequence comparison revealed that the inter-specifc sequence differences were higher: 15.0-34.6% for ITS-1 and 22.7-24.2% for ITS-2 between <I>A. cantonensis </I>and other <I>Angiostrongylus </I>sp. The results showed that the ITS sequences were conserved among the <I>A. cantonensis</I> isolates however, they were quite different from that of other <I>Angiostrongylus </I>species. Therefore, ITS sequences could provide useful genetic markers for the specific identification and genetic characterization of <I>Angiostrongylus </I>sp.}
    }