@article{MAKHILLJAVA20111052632,
    title = {Molecular Cloning and Sequence Characterization and Tissue Transcription Profile Analyses of Three Novel Common Carp (<I>Cyprinus carpio</I>) Genes-<I>RaB7</I>, <I>RaB8a</I> and <I>RDH1l},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {5},
    pages = {585-592},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.585.592},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.585.592},
    author = {BI and},
    keywords = {RDH1L,China,tissue transcription profile,Common carp,RAB7,RAB8A},
    abstract = {The complete coding sequences of three common carp genes <I>RAB7</I>, <I>RAB8A</I> and <I>RDH1L</I> were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) based on the sequence information of the zebrafish and referenced highly homologous common carp ESTs. The sequence analyses of these three genes revealed that common carp <I>RAB7</I> gene encodes a protein of 204 amino acids which has high homology with the RAB family member rab-7 (RAB7) of fifteen species: Atlantic salmon (97%), zebrafish (91%), sheep (90%), western clawed frog (89%), red jungle fowl (90%), African clawed frog (88%), human (89%), dog (89%), horse (89%), <I>Sumatran orangutan</I> (89%), cattle (89%), rat (89%), mouse (89%), <I>Aiptasia pulchella</I> (88%) and rabbit (87%). The common carp <I>RAB8A</I> gene encodes a protein of 207 amino acids which has high homology with the cmember RAS oncogene family rab8a (RAB8A) of eight species: zebrafish (98%), human (92%), dog (92%), cattle (92%), mouse (91%), rat (91%), <I>Sumatran orangutan</I> (91%) and chicken (87%). The common carp <I>RDH1L</I> gene encodes a protein of 319 amino acids that has high homology with tthe retinol dehydrogenase 1, like (RDH1L) of two species: zebrafish (83%) and Atlantic salmon (65%). Phylogenetic tree analysis revealed that the common carp RAB8A and RDH1L have closer genetic relationships with the zebrafish RAB8A and RDH1L but the common carp RAB7 has a closer genetic relationship with the RAB7 of Atlantic salmon. The tissue transcription profile analyses indicated that that the common carp <I>RAB7</I>, <I>RAB8A</I> and <I>RDH1L</I> genes are generally but differentially expressed in the detected tissues including in tissues including muscle, heart, brain, skin, gills, eye, fin. These data serve as a foundation for further research on these three genes.}
    }