@article{MAKHILLJAVA201110243133,
    title = {Simultaneous Detection and Virulence Characterisation of <I>Dichelobacter nodosus</I> from Ovine Footrot by Multiplex PCR},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {24},
    pages = {3192-3195},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.3192.3195},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.3192.3195},
    author = {A.K.,M.M.,B.,S. and},
    keywords = {Footrot,Gelatin gel test,intA gene,multiplex PCR,16S rRNA,India},
    abstract = {Out of 262 swab samples from feet of clinically footrot affected sheep from Jammu and Kashmir, 135 (52.0%) detected positive for <I>Dichelobacter nodosus</I> by <I>16S rRNA</I> gene specific PCR. Out of these135 positive samples, 82 (61.74%) tested positive for <I>intA</I> gene carried by virulent strains. A mulplex PCR for simultaneous detection and virulence characterization of <I>D. nodosus</I> in clinical samples was therefore devised. The test detected 77 (57.03%) samples positive for virulent <I>D. nodosus</I>. The results were comparable and the success of the multiplex PCR was established. Out of 30 randomly selected isolates subjected to gelatin gel test, 24 isolates with <I>intA </I>gene produced thermostable protease while six isolates without <I>intA</I> gene revealed the production of thermolabile protease. This indicated a good co-relation between presence of <I>intA</I> gene and gelatine gel test in determination of the<I> D. nodosus</I> virulence.}
    }