@article{MAKHILLJAVA201110233118,
    title = {<I>S. enteritidis</I> Genome Loads in the Tissues of Reproductive Organs in Laying Duck},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {23},
    pages = {3165-3168},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.3165.3168},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.3165.3168},
    author = {Guang-Zhi and},
    keywords = {Salmonella enteritidis,reproductive organs,duck,PCR,China},
    abstract = {The objective of this study was to determine the replication kinetics of <I>Salmonella enteritidis</I> genome loads (<I>S. enteritidis</I>) in reproductive organs of laying duck after oral challenge. We conducted serovar-specific real-time PCR for <I>S. enteritidis</I> to detect the genomic DNA of <I>S. enteritidis</I> in laying duck at different time points. To validate these results, the Indirect Fluorescent Antibody (IFA) technique was employed too. The results showed that <I>S. enteritidis</I> was consistently detected in all the samples. However, the number of bacteria started decreasing by 3-4 days and by 6 days, the concentration of <I>S. enteritidis</I> DNA was below the detection limits of the PCR assay except the spleen and vagina. In conclusion, this study will help in understanding the pathogenesis of <I>S. enteritidis</I> infection <I>in vivo</I>.}
    }