@article{MAKHILLJAVA201110223053,
    title = {Optimal Method of Mouse Blastomere Biopsy: <I>In vitro</I> Developmental Potential of the Biopsied Embryo to Blastocyst Stage after Aspirated Eight-Cell Mouse Embryos},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {22},
    pages = {3027-3030},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.3027.3030},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.3027.3030},
    author = {Ali,Tolga,Arzu,Sezen and},
    keywords = {Blastomere,embryo biopsy,mouse embryos,micromanipulation,hormones,biopsy group},
    abstract = {The researchers investigated the effect of blastocyst development and quality of blastomere aspiration techniques on eight-cell mouse embryos. The results clearly indicate that the <I>in vitro</I> development of biopsied mouse embryos depended on the suitable aspiration method. This method related to pre-implantation genetic diagnosis for the genesis of animal models for animal disorders from embryos. In this study, female CB6 F1(Balb/cXC57bl/j) hybrid mice were superovulated with hormones and superovulated females were sacrified approximately 68 h after hCG administration. About eight-cell embryos were recovered from oviducts of sacrified mouse in M2 medium. Before biopsy, all embryos were incubated to decrease cell to cell contacts to microdrops of Ca<SUP>2+</SUP>/Mg<SUP>2 +</SUP> free QAM HTF (3 mg mL<SUP>-1</SUP> BSA Fraction V) with HEPES for 90 min at 37&deg;C. A single blastomere of eight-cell embryos were aspirated by the aspiration pippetes under inverted microscope (4X). After biyopsy, embryos were cultured in SAGE medium supplemented with 5% CO<SUB>2</SUB>, 5% O<SUB>2</SUB> and 90% air at 37&deg;C for up to expanded blastocyst stage. After culture, 267 biopsied embryos out of 234 (88.32&plusmn;7.5%) expanded blastocysts developed from the biopsy group and total cell number mean 67.8&plusmn;17.2% were recorded in the experiment group and 126 non-biopsied embryos out of 118 (94.4&plusmn;7.78%) expanded blastocysts developed from control embryos and total cell number mean 70&plusmn;15.4% was recorded in the control group. In the present study, there was no difference in blastocysts developmental rates at post biopsy group and control group (p = 0.05). In conclusion, the biopsy the method described here is an optimal method of blastomere aspiration on <I>in vivo</I> mouse embryos.}
    }