@article{MAKHILLJAVA201110223049,
    title = {Association among Leptin Receptor Gene (<I>LEP-R</I>) with Carcass Weight and Dorsal Fat in a Commercial Type Swine Population from Mexico},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {22},
    pages = {3006-3009},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.3006.3009},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.3006.3009},
    author = {Victor,Priscila,Diana,Rogelio,Juan,Jose,Ramiro and},
    keywords = {Ryanodin receptor,leptin receptor,production traits,swine,genotype,Mexico},
    abstract = {The goal of the present study was to determine by molecular methods the existence of an association among alleles of the Leptin Receptor gene (<I>LEP-R</I>) and dorsal fat in pigs, taking as a fixed effect the genotype for the ryanodyn receptor gene (<I>Ryr-1</I>). Genotyping for both genes was achieved by Polymersase Chain Reaction-restriction Fragment Longitude Polymorphism (PCR-RFLP) using specific PCR primers and restriction enzymes. Polymorphisms of LEP-R were determined by amplification of a 2 kilobase (kb) pair DNA fragment and digestion with restriction enzyme Hpa II whereas for <I>Ryr-1</I> gene the PCR product is a 659 base pair (bp) fragment followed by digestion with enzyme Bis HKA I. A total 192 animals were analyzed (F1) not including the parental 4 males and 26 females. For <I>Ryr-1</I> gene it was found a distribution of 75.7% dominant homozygous (normal), 22.6% heterozygous (normal carrier) and 1.7% recessive homozygous (affected). Allele frequencies were 0.87 N (normal) and 0.13 n (mutant). For <I>LEP-R</I> gene the results indicate 3.2% AA, 29.5% for AB and 67.2% for BB. Allele frequencies were 0.18 A (negative for RFLP), 0.82 B (positive for RFLP). Statistical analyses indicate that LEP-R genotype had an influence on dorsal fat deposition in the studied population.}
    }