@article{MAKHILLJAVA201110203009,
    title = {Molecular Cloning, Sequence Identification and Tissue Expression rofile of Three Novel Sheep (<I>Ovis aries</I>) Genes-<I>RAB1A</I>, <I>RAB4A</I> and<I> RAB5A</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {20},
    pages = {2659-2665},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.2659.2665},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.2659.2665},
    author = {Xia,Liu and},
    keywords = {Sheep,RAB5A,RAB4A,RAB1A,tissue expression,China},
    abstract = {The complete coding sequences of three sheep genes-<I>RAB1A</I>, <I>RAB4A</I> and <I>RAB5A</I> were amplified using the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Sequence analysis revealed that the sheep <I>RAB1</I>A gene encodes a protein of 204 amino acids that shares high homology with the RAB1A, member RAS oncogene family (RAB1A) proteins of nine species-human (98%), mouse (98%), rat (98%), pig (98%), zebrafish (98%), Western clawed frog (97%), Atlantic salmon (96%), disc abalone (92%) and great pond snail (87%). The sheep <I>RAB4A</I> gene encodes a protein of 218 amino acids that shares high homology with the RAB4A, member RAS oncogene family (RAB4A) proteins of eight species-cattle (100%), dog (99%), human (99%), rhesus monkey (99%), horse (99%), chicken (99%), rat (98%) and mouse (98%). The sheep <I>RAB5A</I> gene encodes a protein of 215 amino acids that shares high homology with the RAB5A, member RAS oncogene family (RAB5A) proteins of fifteen species-cattle (98%), rabbit (98%), dog (98%), horse (98%), pig (98%), sumatran orangutan (98%), human (97%), mouse (97%), rat (97%), chimpanzee (97%), chicken (95%), rhesus monkey (93%), African clawed frog (93%), Western clawed frog (93%) and zebrafish (92%). Finally, these three novel sheep genes were assigned to GeneIDs: 100302065, 100302066 and 100302084. Phylogenetic analysis indicated that the sheep <I>RAB1A</I> gene has a closer genetic relationship with the <I>RAB1A</I> genes of human, mouse and rat. The sheep <I>RAB4A</I> and <I>RAB5A</I> genes both have closer genetic relationships with the <I>RAB4A</I> and <I>RAB5A </I>genes of cattle. Tissue expression profile analysis was also carried out and results demonstrated that sheep <I>RAB1A</I>, <I>RAB4A</I> and <I>RAB5A</I> genes were all generally but differentially expressed in detected tissues.}
    }