@article{MAKHILLJAVA201110182979,
    title = {Porcine IFN-&#8226; is Biologically Active and Secreted in the Yeast <I>Pichia 
  pastoris</I> Expression System},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {18},
    pages = {2450-2455},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.2450.2455},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.2450.2455},
    author = {Dongsheng,Zhangyong,Xiaoli,Ming,Kangning and},
    keywords = {Porcine interferon-• (PoIFN-•),Pichia pastoris,expression,activity,cells,biologically active},
    abstract = {Porcine Interferon (PoIFN) is a multifunctional secretory 
  protein produced by animal immune cells. IFN signaling activates host cells 
  resulting in anti-viral and -tumor immune regulation in addition to other biological 
  functions. Here, the coding region of PoIFN-&#8226; was amplified from porcine 
  genomic DNA by Polymerase Chain Reaction (PCR) and was cloned into the yeast 
  <I>Pichia pastoris</I> expression system. This resulted in high levels of gene 
  expression. Furthermore, expression of PoIFN-&#8226; was successfully detected 
  by Western-blot. The relative molecular weight of the product was 25 and 27 
  kDa. The recombinant PoIFN-&#8226; exhibited cross-species antiviral activity 
  in Marc-145 and BHK-21 mammalian cell lines. In summary, the results demonstrate 
  that recombinant PoIFN-&#8226; cloned into <I>P. pastoris</I> is expressed, 
  secreted and biologically active in mammalian cells.}
    }