@article{MAKHILLJAVA201110172929,
    title = {Polymorphic Study of FecX<SUP>G</SUP>, FecG<SUP>H </SUP>and Fec<SUP>B</SUP> Mutations in Four Domestic Sheep Breeds in the Lower Yellow River Valley of China},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {17},
    pages = {2198-2201},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.2198.2201},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.2198.2201},
    author = {Yuan-Fang,Chuan-Sheng,Zheng-Zhu,Li-Ying,Li-Xin,Min-Shan and},
    keywords = {FecGH,China,mutation,prolificacy,sheep,FecB,FecXG},
    abstract = {Genetic mutations with major effects on ovulation rate in sheep were recently identified in two genes of the Transforming Growth Factor (TGF<SUB>&#946;</SUB>) superfamily and a TGF<SUB>&#946;</SUB> receptor, namely BMP15, GDF9 and BMPRIB. The FecX<SUP>G</SUP>, FecG<SUP>H </SUP>and Fec<SUP>B </SUP>were performed to screen four sheep breeds (small tailed Han, Wadi, big tailed Han and Shandi sheep) distributed along the lower Yellow river valley of China by forced PCR-RFLP method if these genes are responsible for their high prolificacies. The Fec<SUP>B</SUP> mutation was found in small tailed Han and Wadi sheep but absent in big tailed Han and Shandi sheep. The 154 small tailed Han sheep included all three genotypes (Fec<SUP>B</SUP>/Fec<SUP>B</SUP>, Fec<SUP>B</SUP>/Fec<SUP>+ </SUP>and Fec<SUP>+</SUP>/Fec<SUP>+</SUP>) at frequencies of 0.54, 0.40 and 0.06, respectively whereas the samples of 30 Wadi sheep were only detected two genotypes, 4 were heterozygous Fec<SUP>B</SUP>/Fec<SUP>+</SUP> and 26 Fec<SUP>+</SUP>/Fec<SUP>+</SUP>. The frequency of the Fec<SUP>B</SUP> allele in small tailed Han and Wadi sheep were 0.74 and 0.07, respectively. Results indicated that the Fec<SUP>B</SUP> mutation is not fixed in two sheep population. The same FecX<SUP>G</SUP> mutation of the <I>BMP-15</I> gene was only found in small tailed Han ewes as in Belclare and Cambridge ewes, frequencies of genotypes FecX<SUP>G+</SUP>, FecX <SUP>++</SUP> and FecX<SUP>GG</SUP> were 0.70, 0.27 and 0.03, respectively. There was no evidence of FecG<SUP>H</SUP> in any of the breeds sampled. The discovery of the Fec<SUP>B</SUP> mutation in small tailed Han sheep and Wadi sheep will facilitate the use of Fec<SUP>B</SUP> allele in improving the prolificacy of non-prolific sheep breeds of China.}
    }