@article{MAKHILLJAVA201110162886,
    title = {A Simpler Method for the Efficient and Precise Deletion of Genes in<I> Salmonella </I>sp.},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {16},
    pages = {2090-2094},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.2090.2094},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.2090.2094},
    author = {Zhi-Fa,Xiang,Shi-Zhong,Qiang,Zhi-Ming and},
    keywords = {temperature,Salmonella,deletion of gene,prgH genehomologous recombination,China},
    abstract = {A simpler method for the efficient and precise deletion of genes in <I>Salmonella </I>sp. was developed. To demonstrate this approach, the <I>prgH </I>gene of <I>Salmonella typhimurium </I>SL7207 was deleted by homologous recombination with a temperature-sensitive plasmid containing a cassette that two DNA fragments as homologous arms flanking chloramphenicol resistance gene (<I>Cm<SUP>R</SUP></I>) which replaced the <I>prgH </I>gene. During screening mutant at 44&deg;C, the temperature-sensitive plasmid was lost easily only the mutant which<I> prgH</I> gene was replaced by <I>Cm<SUP>R</SUP></I> gene could grow in the LB media with chloramphenicol. The results showed that the method was simpler, more effective to delete target gene in genomic DNA of<I> Salmonell </I>sp. than those conventional methods.}
    }