@article{MAKHILLJAVA201110142866,
    title = {Effects of Interferon-Tau (IFN-τ) and Progesterone on Transcription Level of Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) in Cultured Bovine Endometrial Cells},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {14},
    pages = {1833-1840},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.1833.1840},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.1833.1840},
    author = {Song-Jia,Jie,Wei,Si-Yi,Jia,Ming and},
    keywords = {bovine endometrium,TIMP-2,progesterone,IFN-Ä,MMP-2,China},
    abstract = {Interferon-tau (IFN-&#964;) secreted by blastular trophectoderm was thought to be the primary signal for pregnancy recognition during bovine early pregnancy period after pregnancy recognition, progesterone dominated pregnancy maintaining. Moreover, Matrix Metalloproteinases-2 (MMP-2) and Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) was important for embryo implantation. The objectives of studies were evaluated whether IFN-&#964; and progesterone regulated MMP-2 and TIMP-2 expression in cultured bovine Endometrial Cells (bECs). In the studies, bECs were cultured in DMEM-Ham&#146;s F12 medium with free-serum. These cultured cells were divided into 4 groups and cultured in four types culture medium: culture medium without IFN-&#964; and progesterone (Co group); culture medium including 100 ng mL<SUP>-1</SUP> IFN-&#964; (I<SUB>N</SUB> group); culture medium including 200 nmol L<SUP>-1</SUP> progesterone (Pr group); culture medium including 100 ng mL<SUP>-1</SUP> IFN-&#964; and 200 nmol L<SUP>-1</SUP> progesterone (I+P group). Cultured bECs were harvested and extracted total mRNA after being cultured for 1, 3, 6 and 12 h and then expression level of MMP-2 and TIMP-2 were assessed with fluorescence quantitative PCR. The results showed that MMP-2 expression level in Pr, I<SUB>N</SUB> and I+P group were extremely significantly higher than in Co group (p&lt;0.01). MMP-2 expression level in I<SUB>N</SUB> and I+P group was significantly higher than in Pr group (p&lt;0.05). MMP-2 expression level was no statistical difference between I<SUB>N</SUB> group and I+P group (p&gt;0.05); TIMP-2 expression level in Pr, I<SUB>N</SUB> and I+P group was extremely significantly lower than in Co group (p&lt;0.01) and TIMP-2 expression level was no statistical difference among Pr, I<SUB>N</SUB> and I+P group (p&gt;0.05). The conclusion indicated progesterone and IFN-&#964; induced MMP-2 expression <I>in vitro</I> cultured bECs whereas, progesterone and IFN-&#964; suppressed TIMP-2 expression <I>in vitro</I> cultured bECs.}
    }