@article{MAKHILLJAVA201110132814,
    title = {Serotyping <I>Dichelobacter nodosus</I> with PCR-SSCP},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {13},
    pages = {1678-1682},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.1678.1682},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.1678.1682},
    author = {Jon and},
    keywords = {Single Strand Conformational Polymorphism (SSCP),PCR,fimA gene,Dichelobacter nodosus,sequencing,sample},
    abstract = {In this study, we reported rapid approach for identification of <I>D. nodosus</I> serotypes that causes footrot, higly contagious diseases of the ruminants in worldwide with using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) analysis. A total of 500 swab samples from infected hoof lesion were investigated for presence of <I>D. nodosus</I> by PCR amplification of <I>fimA</I> gene. PCR-SSCP patterns revealed that each sample contained two or three different <I>D. nodosus</I> serotypes. Additionally on the of basis SSCP patterns, 8 different serogroups (A- G and I) were identified from New Zealand samples. These findings were confirmed by cloning and sequences of <I>fimA</I> gene.}
    }