@article{MAKHILLJAVA201110122843,
    title = {Polymorphisms and Sequencing Analysis in 5'Upstream and Intron 2 Region of <I>H-FABP</I> Gene in Anhui Wannan Black Pig Population},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {10},
    number = {12},
    pages = {1568-1572},
    year = {2011},
    issn = {1680-5593},
    doi = {javaa.2011.1568.1572},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2011.1568.1572},
    author = {Zongjun,Chenghua,Xiaoling,Xiaolei,Long and},
    keywords = {sequencing,China,PCR-RFLP,IMF content,H-FABP,Wannan black pig},
    abstract = {In order to give a basis for molecular breeding of meet quality traits in Wannan black pig, we applied PCR-RFLP method to analyze the polymorphism of 5'upstream region and intron 2 of <I>H-FABP</I> gene in 184 Wannan black pigs population. The results show that there exist polymorphic loci of restriction enzyme Hinf in 5'upstream region and Hae III as well as Hinf I in intron 2. Except for locus of restriction enzyme HinfI, the other two polymorphism sites achieved in Hardy-Weinberg equilibrium based on &#967;<SUP>2</SUP>-test (p&gt;0.05). In addition, a T&rarr;C mutation in 1324 bp, a C&rarr;G mutation in 1811 bp and a C&rarr;T mutation in 1970 bp were found through the direct sequencing the polymorphic RFLP fragments of <I>H-FABP</I> gene, then genotype frequencies, gene frequencies, H<SUB>o</SUB>, H<SUB>e</SUB>, N<SUB>e</SUB> and PIC were investigated in Wannan black pig population. The results identified 3 variant restriction sites and provided a basic data for further study of the relationship between IMF and H-FABP with different genotypes and also provide basic for make sure whether <I>H-FABP</I> gene is the key gene for effect on the deposition of IMF.}
    }