@article{MAKHILLJAVA2010942078,
    title = {Introduction of Exogenous Gene into Chicken PGCs via Blastoderm},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {9},
    number = {4},
    pages = {748-750},
    year = {2010},
    issn = {1680-5593},
    doi = {javaa.2010.748.750},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2010.748.750},
    author = {T.,R.,T. and},
    keywords = {blastoderm, PGCs,gene transfer,Chicken embryo,exogenous gene,Lac Z},
    abstract = {It was proved that an exogenous gene was successfully transferred to chicken embryo and Primordial Germ Cells (PGCs) by the injection of the blastoderm. In this experiment, an exogenous gene, Lac Z constructs encoding <I>Escherichia coli</I> beta-galactosidase was introduced into chicken blastoderm (stage X) just ovoposition using lipofection reagent. After injection of gene the eggs were incubated in routine manner until developmental stage 12-15. Survival rate of the treated embryo was 35.6%. Lac Z expression was mosaic manner and low efficiency. However, it was obtained Lac Z specific band from chicken PGCs by PCR method.}
    }