@article{MAKHILLJAVA2010942068,
    title = {Field Evaluation of PCR Assays for the Diagnosis of Tropical Theileriosis in Cattle and Water Buffaloes in Egypt},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {9},
    number = {4},
    pages = {696-699},
    year = {2010},
    issn = {1680-5593},
    doi = {javaa.2010.696.699},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2010.696.699},
    author = {X.Q.,A.M.,M.F.,Y.S.,F.A. and},
    keywords = {Theileria annulata,tropical theileriosis,PCR,microscopic examination,diagnosis,Cattle,water buffaloes,Egypt},
    abstract = {The study evaluated the usefulness of specific PCR assays for the diagnosis of tropicaltheileriosis caused by <I>Theileria annulata</I> in Egypt using Microscopic Examination (ME) for comparison. Blood samples from 258 animals comprising both sick and apparently healthy cattle and water buffaloes were examined for the presence of <I>Theileria</I> infection by ME using Giemsa-stained blood smears and the prevalence was 18.6% (48/258). To evaluate the usefulness of PCR assays for the identification <I> Theileria</I> sp. and <I>T. annulata</I>, 30 bovine blood samples selected from the 258 animals were tested by both PCR assays and ME and ME identified 9/30 (30%), whereas the PCR assay for <I>Theileria</I> sp. was more sensitive which identified 21/30 (70%). Of these 21 <I>Theileria</I> positive samples, 15 were identified as infected with <I>T. annulata</I> by specific PCR assay for <I>T. annulata</I>. This study demonstrated that specific PCR assays are more sensitive and accurate for the clinical diagnosis of tropical theileriosis.}
    }