@article{MAKHILLJAVA20109242525,
    title = {Detection of <I>Mycobacterium avium</I> Subspecies <I>paratuberculosis</I> in Milk and fecal Samples in Dairy Cattle by PCR and Nested-PCR},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {9},
    number = {24},
    pages = {3055-3061},
    year = {2010},
    issn = {1680-5593},
    doi = {javaa.2010.3055.3061},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2010.3055.3061},
    author = {A.,M. Tohidi,S. and},
    keywords = {feces,single PCR,paratuberculosis,milk,Mycobacterium avium,subsp,nested-PCR,Cattle},
    abstract = {<I>Mycobacterium avium</I> ssp. <I>paratuberculosis</I> (MAP) is the etiologic agent of Johne&#146;s disease in cattle. The disease causes diarrhea, reduced milk production, poor reproductively, infertility and emaciation. MAP was isolated from intestinal tissue of a human patient suffering from Crohn's disease, a controversial discussion exists whether MAP have a role in the etiology of Crohn's disease or not. Milk may be a potential vehicle for the transmission of MAP to human population. In this study, milk and fecal samples were collected from cows (n = 150) with suspected of Johne&#146;s status. Milk and fecal samples were processed for Nested PCR. Nested PCR detected positive samples in milk and feces 68.150 (45.3%) and 74.150 (49.3%), respectively and PCR detected positive samples in milk and feces 41.150 (27.3%) and 48.150 (32%), respectively.}
    }