@article{MAKHILLJAVA20109242510,
    title = {Expression of Interferon-Stimulated Gene 15 in Endometrial Explants from Early Pregnant Cows <I>in vitro</I> for 60 Days},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {9},
    number = {24},
    pages = {3016-3020},
    year = {2010},
    issn = {1680-5593},
    doi = {javaa.2010.3016.3020},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2010.3016.3020},
    author = {Le-Ying,Jian-Guo,Ling and},
    keywords = {ISG1 5,primary experiment,in vitro,bovine,Endometrium,pregnant cow},
    abstract = {Interferon-tau is generally regarded as the primary signal for maternal recognition in ruminant animals. Expression of interferon-stimulated gene 15 (ISG15) is up-regulated in bovine endometrium during early pregnancy. The experimental materials were from our primary experiment. Tissue explants of endometria from day 18 of pregnant and non-pregnant cows were cultured <I>in vitro</I> for 60 days and then the expression of ISG15 in these explants was detected by western blot. The results indicated that ISG15 and conjugated proteins were still expressed at a high level in endometrial explants from pregnant cows after cultured <I>in vitro</I> for 60 days and there was no expression in endometrial explants from non-pregnant cows after cultured <I>in vitro</I> for 60 days. In conclusion, expression of ISG15 in endometrial explants from early pregnant cows maintained <I>in vitro</I> for a long time.}
    }