@article{MAKHILLJAVA20109212462,
    title = {Using Bioinpormcotics Methods to Develop EST-SSR Makers from Sheep’s ESTs},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {9},
    number = {21},
    pages = {2759-2762},
    year = {2010},
    issn = {1680-5593},
    doi = {javaa.2010.2759.2762},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2010.2759.2762},
    author = {Wenxiang,Zunbao,Zongsheng,Xiancun,Hongbin and},
    keywords = {Sheep,EST,SSR,NCBI,genetic mapping,China},
    abstract = {About 29685 ESTs of sheep’s skin from NCBI were mined to develop EST-SSR markers. Totally, 1456 SSRs were identified from 1364 ESTs using SSRHunter soft tools. The frequency of EST-SSRs was 4.9%. The dinucleotide repeat motif was the most abundant SSR accounting for 32.14% followed by 28.23, 7.14, 25.21 and 7.28%, respectively for tri, hexa, penta and tetra-nucleotide repeats. Among the di-nucleotide repeats, AC/TG (58.33%) were the most abundant type. About 261 primer pairs were designed from microsatellite among which 62 pairs could show clear PCR products by electrophoresisand 5 of these primers revealed moderate to high Polymorphism Information Content (PIC) in Chinese Merino sheep. The mean PIC value was 0.6971, ranging from 0.5499-0.7440.}
    }