@article{MAKHILLJAVA20109212448,
    title = {The Polyclonal Antibody Against Chicken Interleukin-6 Prepared by Immunization of Recombinant Eukaryotic Plasmid Can React Efficiently with its Prokaryotic Expression Protein},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {9},
    number = {21},
    pages = {2679-2684},
    year = {2010},
    issn = {1680-5593},
    doi = {javaa.2010.2679.2684},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2010.2679.2684},
    author = {Min,Yuanping,Nianli,Sanjie,Xintian and},
    keywords = {Chicken IL-6,eukaryotic expression,prokaryotic expression,polyclonal antibody,Western-blot,indirect ELISA},
    abstract = {Interleukin-6 (IL-6) is a multifunctional cytokine which has a wide range of activity in animals and has an impact on almost all immune system cells. To produce specific antibody against chicken IL-6 and detect its activity, its complete Open Reading Frame (ORF) sequence was amplified and inserted into the eukaryotic expression vector pcDNA3.1 (+) to get recombinant eukaryotic plasmid pcDNA-ChIL-6 and BALB/c mouse were immunized with this plasmid to produce polyclonal antibody against Chicken IL-6. Then the mature protein coding genes of chicken IL-6 was cloned and inserted into the prokaryotic expression vector pET-32a (+) to get recombinant prokaryotic plasmid pET-ChIL-6, the expression of this fusion protein was successfully induced by Isopropy 1-β-D-thiogalactoside (IPTG) and the recombinant protein was further purified by NTA His•Bind column. The purified recombinant protein could react positively with polyclonal antibody against pcDNA-ChIL-6 in Western-blot and indirect ELISA.}
    }