@article{MAKHILLJAVA20109182393,
    title = {Sequences of Internal Transcribed Spacers and Two Mitochondrial Genes: Effective Genetic Markers for <I>Metorchis orientalis</I>},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {9},
    number = {18},
    pages = {2371-2376},
    year = {2010},
    issn = {1680-5593},
    doi = {javaa.2010.2371.2376},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2010.2371.2376},
    author = {X.Q.,Y.C.,J.,M.X.,H.,X.N.,S.H.,Y.N.,L. and},
    keywords = {cytochromec oxidase subunit 1 (cox1),mitochondrial DNA (mtDNA),Internal Transcribed Spacers (ITS),trematodes,Opisthorchiidae,metacercaria,Metorchis orientalis,NADH dehydrogenase subunits 1 (nad1),phylogenetic analysis,genetic marker},
    abstract = {The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) and two mitochondrial DNA (mtDNA) regions, namely cytochromec oxidase subunit 1 (cox1), NADH dehydrogenase subunits 1 (nad1), among <I>Metorchis orientalis</I> metacercaria isolates from Guangxi in China. The sequences of ITS, pcox1 and pnad1 were amplified from 6 individual <I>M. orientalis</I> metacercariae and sequenced. The relevant sequences of other 7 trematode species belonging to 6 genera in 4 families were downloaded from GenBank and their phylogenetic relationships were re-constructed using the combined pcox1 and pnad1 mt DNA sequences with <I>Trichinella spiralis</I> as outgroup. The results showed that sequences of ITS rDNA, pcox1 and pnad1 of <I>M. orientalis</I> were 1131, 654 and 650 bp, respectively and they were quite conserved among the <I>M. orientalis</I> isolates. However, they were quite different from that of other species, phylogenetic analysis of the combined pcox1 and pnad1 mt DNA sequences were able to distinguish <I>M. orientalis</I> from different species of the <I>Opisthorchiidae</I> and trematodes in other families. Therefore, the ITS, cox1 and nad1 mt DNA sequences provide effective genetic markers for the specific identification of trematodes of the <I>Opisthorchiidae</I> family and have implications for studying their population biology, genetic structure, as well as molecular epidemiology.}
    }