@article{MAKHILLJAVA20109152325,
    title = {The Study of <I>PIT1</I> Gene Polymorphism in the Najdi Cattle Using PCR-RFLP Method},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {9},
    number = {15},
    pages = {2001-2003},
    year = {2010},
    issn = {1680-5593},
    doi = {javaa.2010.2001.2003},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2010.2001.2003},
    author = {S.,J.,T.,Z. and},
    keywords = {growth harmone,allele,najdi cattle,polymorphism,PIT1 gene,prolactin},
    abstract = {The part of the bovine genome which shows a superior action and explains the major part of variation of the economical production traits were known as QTL. <I>PIT1</I> gene which is also termed hormone factor-1 is a pituitary-specific transcription factor which has responsible for pituitary development and hormone expression in mammals. The main function of <I>PIT1</I> is for binding and trans-activity promoters of both Growth Hormone (GH) and Prolactin (PRL) gene. It was subjected to different molecular studies as a key role for understanding genetic variation in dairy cattle. This study was aimed to detect the polymorphism in a part of <I>PIT1</I> gene, exon 6 in Najdi cattle. DNA was extracted from blood sample collected from 84 Najdi Cattle and submitted for Polymerase Chain Reaction (PCR) followed by digestion with <I>HinfI</I> restriction enzyme. Base on PCR-RFLP method, it found that the frequency of the A and B alleles of this gene for Najdi Cattle were 18.45 and 81.55%, respectively. The genotypic frequencies of AA, AB and BB were 3.57, 29.76 and 66.66%, respectively. &#967;<SUP>2</SUP>-test indicated that these two populations were at Hardy-Weinberg equilibrium. Thus, the selection base on these loci in the population is possible to be done.}
    }