@article{MAKHILLJAVA20109112236,
    title = {Effect of Legume (<I>Phaseolus calcaratus</I>) Hay Supplementation on Rumen Cellulolytic Bacterial Populations in Swamp Buffaloes Investigated by the Real-Time PCR Technique},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {9},
    number = {11},
    pages = {1654-1659},
    year = {2010},
    issn = {1680-5593},
    doi = {javaa.2010.1654.1659},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2010.1654.1659},
    author = {Metha and},
    keywords = {evalute,supplementation,fed legumes,swamp buffalo,Cellulolytic bacteria,determinate,Thailand},
    abstract = {A quantitative real-time PCR approach was used to determine the population densities of major ruminal cellulolytic bacterial species (<I>Fibrobacter succinogenes</I>, <I>Ruminococcus albus</I> and <I>Ruminococcus flavefaciens</I>) in rumen fluid and digesta of swamp buffalo (<I>Bubalus bubalis</I>). Four rumen-fistulated, male swamp buffalo were randomly assigned according to a 4x4 Latin square design to evaluate the effect of <I>Phaseolus calcaratus</I> Hay (PCH) supplementation. PCH contained 18.3% Crude Protein (CP) and 2.8% condensed tannins. Animals were given 0, 300, 600 and 900 g day<SUP>-1</SUP> as supplements. All animals were given <I>ad libitum</I> access to rice straw while additional concentrate (12.6% CP) was given at 0.3% body mass and each period lasted for 21 days. At the end of each period, rumen fluid and digesta was collected at 0, 4 h post morning feeding. It was found that PCH supplementation increased these three cellulolytic bacteria <I>F. succinogenes</I> between 2.5 and 5.5x10<SUP>9</SUP>, <I>R. flavefaciens</I> between 3.6 and 9.1x10<SUP>9</SUP>, <I> R. albus</I> between 5.7 and 17.9x10<SUP>8</SUP> copies mL<SUP>-1</SUP> at 0, 300, 600 and 900 g day<SUP>-1</SUP> of supplementation, respectively. Moreover, at 4 h post-morning feeding, the populations of the two cellulolytic bacteria were higher than those found at 0 h post-morning feeding. It is most notable that <I>R. flavefaciens</I> and <I>R. albus</I> were the highest in population in the rumen of swamp buffalo, hence indicating high ability in utilizing high fibrous feeds.}
    }