@article{MAKHILLJAVA20109112233,
    title = {Characteristics of Proliferation and Differentiation-Dependent C/EBP, PPARγ and Leptin Gene Expression Patterns Intrinsic to Bovine Intramuscular, Perirenal and Subcutaneous Preadipocytes},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {9},
    number = {11},
    pages = {1639-1645},
    year = {2010},
    issn = {1680-5593},
    doi = {javaa.2010.1639.1645},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2010.1639.1645},
    author = {Makiko,Yukio,Koichiro and},
    keywords = {Adipose depotsite,C/EBP,differentiation,gene expression,leptin,PPAR³,preadipocyte,proliferation},
    abstract = {Adipose depotsite-specific variability in physiological characteristics of preadipocytes could be in part, intrinsic to the cells and become evident through <I>in vitro</I> proliferation and differentiation-dependent gene expression patterns in preadipocyte in the cell culture. This study was designed to characterize proliferation and differentiation-dependent gene expression patterns intrinsic to intramuscular, perirenal and subcutaneous preadipocytes. About 3 type preadipocytes are prepared using the ceiling culture method from bovine intramuscular, perirenal and are demonstrated subcutaneous adipose depots of a single Japanese Black steer. Adipose depot site-specific proliferation and differentiation-dependent gene expression patterns intrinsic to preadipocyte by comparing the mRNA levels of CCAAT enhancer binding protein C/EBP&#945;, C/EBP&#946;, C/EBP&#948;, Peroxisome Proliferator-Activated Receptor &#947; (PPAR&#947;) and leptin before and after the induction of differentiation among culture of the 3 type preadipocytes. These results may suggest the existence of the site-specific preadipocyte-intrinsic proliferation and differentiation program.}
    }