@article{MAKHILLJAVA200984644,
    title = {Genotyping of Kappa-Casein Locus by PCR-RFLP in Brown Swiss Cattle Breed},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {8},
    number = {4},
    pages = {779-781},
    year = {2009},
    issn = {1680-5593},
    doi = {javaa.2009.779.781},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2009.779.781},
    author = {Unsal Dogru and},
    keywords = {Kappa-casein,PCR-RFLP,genetic marker,polymorphism,brown swiss,cattle},
    abstract = {A Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) test was performed on DNA samples extracted from blood samples of Brown Swiss cattle to detect genotype frequency of the bovine kappa-casein (CNS3) locus. A 351 bp fragment of CSN3 was amplified and digested with Hinf I restriction enzymes. Samples were loaded on agarose jel (3%) and genotyped under UV light. Three genotypes were observed, frequencies were 19.35, 20.43 and 60.22% for AA, BB and AB, respectively. Two genetic variants CSN3 A and CNS3 B were identified and the allelic frequencies were estimated as 0.495 and 0.505, respectively. It was concluded that the population was in Hardy-Weinberg equilibrum.}
    }