@article{MAKHILLJAVA20098121928,
    title = {Cervical or Intrauterine Artificial Insemination in Pelibuey Ewes, with Chilled Semen},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {8},
    number = {12},
    pages = {2621-2625},
    year = {2009},
    issn = {1680-5593},
    doi = {javaa.2009.2621.2625},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2009.2621.2625},
    author = {Ruben Dario,Lorenzo,Alejandro C.,Angel A.,Javier and},
    keywords = {Pelibuey ewes,insemination,fertility,intrauterine,Mexico},
    abstract = {In order to evaluate the effect of the site of cooled semen deposition (intrauterine or cervical) on subsequent fertility in Pelibuey ewes, a study was carried out during the breeding season in the dry tropic region from the Southern of Mexico, at 18&deg;15´N and 99&deg;38´W.  A total of 40 cycling Pelibuey ewes from 2-4 years of age were used with corporal condition from 2-3, on a scale of 0-5. To synchronize estrus, intravaginal sponges were applied to the females, containing 20 mg of Flourogestone Acetate (FGA) for 11 days, plus 200 IU of equine Corionic Gonadotropine (eCG) administered intramuscularly when, the sponges were removed. Once removed, estrous was detected every 12 h with males fitted with an apron. Of the 40 ewes considered initially, five expelled the sponge and three others did no present estrus, thus 32 were used. Of those that presented estrus, 16 received cervical insemination using cooled semen 12 later and 16 others too were inseminated 12 h later with cooled semen deposited into the uterus by laparoscopy. The semen was collected by artificial vagina from a Dorper ram 12 h  before the start of inseminations  with an artificial vagina and an evaluation was made of its volume (mL), mass motility (percentage) and spermatic concentration (millions of cells mL<SUP>-1</SUP>). For the preparation of the diluents, the following were used: Triladyl (20%), distilled water (60%) and egg yolk (20%). The diluted semen was manually placed into straws with a capacity of 0.25 mL, at 30&deg;C, which  were  sealed  with powdered  polyvinyl  alcohol.   The  sealed  straws  were  placed  in a  plastic container  with  a  water  at  30&deg;C,  which  was  introduced  into  a  cooler  with  ice  cubes,  where, it was maintained at 5&deg;C, until the insemination took place, within the 12-16 h following its collection. The fertility rate of 75% obtained by intrauterine insemination was higher (p&lt;0.05) than that registered for vaginal insemination (50.0%).}
    }