@article{MAKHILLJAVA2009871065,
    title = {Short-Term Chilled Storage of Cat Semen Extended with and Without Taurine Containing Milk Extenders},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {8},
    number = {7},
    pages = {1367-1371},
    year = {2009},
    issn = {1680-5593},
    doi = {javaa.2009.1367.1371},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2009.1367.1371},
    author = {Alper Baran,Kamber Demir,B. Evrim Sahin,Mithat Evecen,Suleyman Bacinoglu,Serhat Alkan and},
    keywords = {Cat,semen,taurine,extender,cooling,storage},
    abstract = {The aim of this study was to observe the effects of taurine containing and non-containing milk extenders on the spermatological characteristics of chilled stored male cat semen. Semen was collected from 4 cats of 2-3 ages under general anesthesia (xylazine-ketamine HCl) by an electro-ejaculator during the study. Total 5 ejaculates with a weekly basis were collected from each male cat. The semen was split into 2 portions and one was extended with taurine containing Skimmed Milk-Glucose (SMG-T) extender at 26&deg;C (egg yolk (10 mL)-D (+) glucose (1.00 g)-taurine (50 mM)-skimmed milk (to 100 mL)-penicillin-G (1000 IU mL<SUP>-1</SUP>)-streptomycin sulphate (1000 &#956;g mL<SUP>-1</SUP>)) the other portion was extended with Skimmed Milk-Glucose extender (SMG) (egg yolk (10 mL)-D (+) glucose (1.00 g)-skimmed milk (to 100 mL)-penicillin-G (1000 IU mL<SUP>-1</SUP>)-streptomycin sulphate (1000 &#956;g mL<SUP>-1</SUP>)). The extended semen samples were chilled to 5&deg;C in an hour (0.35&deg;C min<SUP>-1</SUP>). Spermatological investigations such as motility (%) and abnormal spermatozoon rates (%) (acrosome, head, mid-piece, tail and total) were done at dilution, cooling to 24 h, 48 h and 72 h at 5&deg;C. Morphological defect rates were examined by staining with Spermac&reg; stain by a light microscope at 1000 magnification. The mean motility of cooled semen in the SMG-T extender at the 24 h was the highest with 75.50&plusmn;5.82% and this value was 66.25&plusmn;12.86% in the SMG extender (p&lt;0.05). During the above time period, the mean acrosome and total abnormal spermatozoon rates in the SMG-T extender were lowest with 18.55&plusmn;3.15 and 32.70&plusmn;4.59%, respectively, these averages were highest in the SMG extender with 19.60&plusmn;5.67 and 40.15&plusmn;9.10%, respectively (p&lt;0.05). The motility and morphologic defect rates at the 48 h were similar in the SMG-T and SMG extenders. At the 72 h, acrosome and tail defect rates in the SMG-T extender were lowest with 33.50&plusmn;4.36 and 15.65&plusmn;6.26%, respectively whilst, these values were highest in the SMG extender with 40.45&plusmn;8.76 and 17.65&plusmn;3.84%, respectively (p&lt;0.05). At the conclusion of the study, it can be said that SMG-T and SMG extenders can be successfully employed to store cat semen for shorter periods (24-48 h), also, SMG-T extender is superior to the SMG extender in cooled storage of cat semen for 24 h.}
    }