@article{MAKHILLJAVA200986934,
    title = {Comparing <I>in vitro</I> Embryonic Development of Bovine Oocytes Cultured in G1.3/G2.3 Sequential Culture Media and CR1aa Medium},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {8},
    number = {6},
    pages = {1185-1189},
    year = {2009},
    issn = {1680-5593},
    doi = {javaa.2009.1185.1189},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2009.1185.1189},
    author = {Mesut Cevik,Hakan Sagirkaya,Arzu Tas,Tolga Akkoc,Haydar Bagis and},
    keywords = {Bovine,IVF,embryo culture,G1.3/G2.3 sequential media,CR1aa},
    abstract = {<I>In vitro</I> embryo culture is an important step of <I>in vitro</I> production of bovine embryos. The aim of this study was to compare the <I>in vitro</I> development of bovine embryos in G1.3/G2.3 sequential culture media and CR1aa culture medium. Oocytes were obtained from ovaries of slaughtered cows and matured in TCM-199 medium in humidified air containing 5% CO<SUB>2</SUB> at 39&deg;C. Matured oocytes were fertilized <I>in vitro</I> using frozen bull sperm prepared with Percoll separation. After fertilization, the presumptive zygotes were denuded from cumulus cells and randomly allotted for 2 treatments: Culture in CR1aa (n = 116) and G1.3/G2.3 (n = 125). The embryo culture was carried out in 50 &#956;L droplets of the media that were placed in incubator containing 6% CO<SUB>2</SUB>, 5% O<SUB>2</SUB> and 89% N<SUB>2</SUB>. The embryos were evaluated on days 3, 5 and 7 following <I>in vitro</I> fertilization. The difference of cleavage rates between 2 treatments was not found significant (p&gt;0.05). However, significant difference was observed between 2 treatments at the level of the obtaining rate of morula and blastocyst (p&lt;0.05). The results showed that G1.3/G2.3 sequential media significantly favoured developing of morula and blastocysts in comparison to CR1aa medium (32.0% versus 10.40% versus 18.96 and 2.58%, respectively).}
    }