@article{MAKHILLJAVA200876893,
    title = {The Determination of Incubation Time and Amount of Faecal Content of Horse Faeces as an Inoculum Source for Digestibility Determination of Forages with <I>in vitro</I> Procedure},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {7},
    number = {6},
    pages = {698-702},
    year = {2008},
    issn = {1680-5593},
    doi = {javaa.2008.698.702},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2008.698.702},
    author = {N. Denek,E. Polat,S. Koncagul and},
    keywords = {Horse,faeces,in vitro digestibility},
    abstract = {This study was carried out to determine incubation time and amount of horse fecal content for measuring <I>in vitro</I> dry matter digestibility of roughages (barley straw, wheat straw, lentil straw, wheat silage, corn silage and alfalfa hay) and their comparison with <I>in vivo</I> digestibility values. In <I>in vitro</I> study, inoculums were prepared using 100, 150, 200, 250, 300, 330, 500, 660 ve 1000 g faeces for per liter of buffer. Roughages were incubated with these inoculums at two incubation times (48 vs 72 h) before 48 h pepsin-HCl digestion. Six fat tailed Awassi rams (42.7±2.5 kg) at the age of two years were used as experimental animals (3 per treatments) in an apparent digestibility experiment. According to result of this  study,  the  best  equation  for estimating <I>in vivo</I> DM digestibilities was obtained with usage of inoculum containing 250 g faeces for per liter buffer solution at length of 48 h incubation (r = 0.93, Y = 24.11 (±2.57) + 0.62 (±0.05) X, R<SUP>2</SUP> = 0.87 ve RSD = 3.19) and 660 g faeces for per liter buffer solution at length of 72 h incubation (r = 0.94, Y = 16.08 (±2.88) + 0.72 (±0.05) X, R<SUP>2</SUP> = 0.89 ve RSD = 2.88). Even though, result from this study showed that horse faecal fluid had high potential to be used for predicting <I>in vivo</I> DM digestibility, further researches dealing with larger number of feeds with known <I>in vivo</I> DM digestibility are required to improve usage horse faecal fluid for <I>in vitro</I> inoculums source.}
    }