@article{MAKHILLJAVA200875766,
    title = {Microvesicles of <I>Actinobacillus seminis</I> Show Proteolytic Activity},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {7},
    number = {5},
    pages = {616-619},
    year = {2008},
    issn = {1680-5593},
    doi = {javaa.2008.616.619},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2008.616.619},
    author = {Arturo Jimenez-Pacheco,Alma Nunez-del Arco and},
    keywords = {Actinobacillus seminis,proteases,microvesicles,proleolytic activity},
    abstract = {The objective of the present study was to identify proteolytic activity in microvesicles (MVs) from <I>Actinobacillus seminis</I>. A reference strain <I>A. seminis</I> ATCC 15768 and a field strain isolated were used. The microvesicles were obtained from ultracentrifugation from 48 h cultures. The proteolytic activity was determined in PAGE-SDS (12%) copolimerized with gelatin at 1%. The protein profile of the microvesicles was also established. The size of MVs was determined with transmission electron microscopy and it was determined to range  from  100-150 nm. The protein profile rendered a 75 kDa  protein, previously reported to be specific for <I>A. seminis</I>. Proteolytic activity was detected in the bands located at 79.5, 59, 40, 27 and 13.5 kDa. These results demonstrated the presence of proteolytic activity in <I>A. seminis</I> MVs. Further investigation on protease characterization and assessment of their importance in ovine epididimitis pathogenesis is needed.}
    }