@article{MAKHILLJAVA2008791274,
    title = {Crotalic Venom Fraction as Promoter of the Transfection Mechanism of a Genic Vaccine (Naked DNA) Against Rabies},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {7},
    number = {9},
    pages = {1045-1055},
    year = {2008},
    issn = {1680-5593},
    doi = {javaa.2008.1045.1055},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2008.1045.1055},
    author = {Arcelia Alvarado Islas,Angel Horacio Sandoval Trujillo,Victor R. Tenorio Gutierrez,Rogelio Alonso Morales,Octavio de Paz Villafan and},
    keywords = {Hemoagglutinins,lectins,plasmids,Agkistrodon piscivorus},
    abstract = {The AL27 crotalic fraction is a lectin recognized by cell receptors and then introduced into the cytoplasm. In the present research a new transfection promoter agent for a non viral expression vector was used, using as a model a plasmid expressing the gene that codifies for the G glycoprotein of the rabies virus. The  objective  was to evaluate the ability of AL27 to increase the rabies plasmid transfection in <I>in vitro</I> and <I>in vivo</I> conditions. The AL27 fraction was isolated from the venom of <I>Agkistrodon piscivorus</I> by HPLC-RP. AL27 maintained its recognition by sialic acid receptors up to a titer of 64 HU. The pC38 plasmid was selected from a panel of 18 clones constructed by inserting the gene that codifies for glycoprotein G of rabies strain HQIMSS99 into the commercial pCI-neo expression vector. <I>In vitro</I> results showed that pC38, with or without AL27, transfected CHO cells under the calcium-phosphate system and using Confocal Microscopy (CM) increased fluorescence (+++) was observed with the mixture pC38/AL27, than with pC38 alone (++). Flow cytometry (FC) revealed expression percentages of 27.78 and 38.66% for pC38 and pC38/AL27, respectively. The <I>in vivo</I> experiment was performed using three groups of BALB/C mice: group 1 was inoculated with pC38 in the tibial muscle; group 2 with pC38 and AL27 and group 3, negative control, with only PBS. Tissues showed the highest expression by CM (+++) when using pC38/AL27. Antibodies detected ranged from 0.75-1.2 international units (IU) mL <SUP>1</SUP> for pC38 and from 1.1-1.27 IU mL <SUP>1</SUP> for pC38/AL27. Results showed that AL27 promoted cell entrance mechanisms, generating a better expression level of the gene that codifies for the G glycoprotein of the rabies virus and consequently enhancing the stimulation of the immune response.}
    }