@article{MAKHILLJAVA2007681158,
    title = {<I>In vitro</I> Assessment of Efficacy of Gamma Irradiation on the Antimicrobial Activity of Iranian Honey},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {6},
    number = {8},
    pages = {996-999},
    year = {2007},
    issn = {1680-5593},
    doi = {javaa.2007.996.999},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2007.996.999},
    author = {F. S. Sabet Jalali,A. Ehsani,H. Tajik and},
    keywords = {Gamma irradiation,antibacterial,Iranian honey,25 KGy},
    abstract = {Unprocessed honey is well recognized as wound-healing remedy. However, to make use of the honey clinically acceptable, it should be sterilized. It is well-established that the antibacterial activity is heat-labile either by using sterilization by autoclaving, but the effectiveness of gamma-irradiation on the antibacterial activity of honey is unknown. Therefore, an investigation was carried out to assess the effects of the antibacterial activity of Iranian honey using the commercial gamma-irradiation sterilization procedure. The honeys were divided into 4 groups (0, 5, 15 and 25 KGy). Then each group was divided four sub-groups non-irradiated and irradiated (non-heated, 25ºC, 35ºC, 45ºC). Microbiological test of the honeys were carried out against control organisms (<I>Staphylococcus aureus </I>, <I>Escherichia coli </I>and <I>Pseudomonas aeruginosa</I>) and clinically isolated organisms were  tested in an agar disk diffusion assay. Results showed that there were no significant changes using the antibacterial activity of gamma-irradiation sterilization of honey, even the radiation was 25 KGy. The results of the study indicate that a better antibacterial activity is derived using the gamma-irradiation technique to maintain sterility and produced undesirable effect on antibacterial activity of honey.}
    }