@article{MAKHILLJAVA200762264,
    title = {Transfer of <I>Rhodoccocus equi</I> Immunity in Guinea Pigs by Mean of Sensitized Spleen Cells},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {6},
    number = {2},
    pages = {223-233},
    year = {2007},
    issn = {1680-5593},
    doi = {javaa.2007.223.233},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2007.223.233},
    author = {Khairelsid Abdalla,Tal b Mohamed,Khalifa Khalifa and},
    keywords = {Rhodoccocus equi,guinea pigs,spleen cells,statistical analysis,PBS,DTH},
    abstract = {This  study  was  performed  to  transfer  the  immunity  against <I> R. equi</I> by using sensitized spleen cells  in  guinea  pigs. Firstly,  two  groups  of   guinea   pigs   were   used,   the   first   injected   i.m  with formalin  killed  antigen  prepared   from <I>  R.   equi</I>    mixed   with   in  complete   Fruend’s   adjuvant   at  two weeks  interval.  While  the  second  was  inoculated  with  the  adjuvant  plus Phosphate Buffer Saline (PBS) and  kept  as  control.  The  immune  response,  both  humoral  and  cellular were monitored using tube agglutination  test,  passive  haemagglutination  test,  skin test, E-rosette and migration inhibitory factor in treated  groups.  Injection  of  animals  induced  both  types  of  immune response, the treated and control groups considered as donors for sensitized and non-sensitized spleen cells, respectively. Secondly, three groups were used as recipients, the first one injected i.p with 2 mL of 5X10<SUP>8</SUP> cell mL<SUP>-1</SUP> sensitized spleen cells, the second group similarly injected with non-sensitized cells and the third one injected with PBS only then kept as control. The immune response was monitored in the recipients which revealed specific <I>R. equi</I> antibodies and developed Delayed Type Hypersensitivity (DTH).  Statistical analysis was done using one way analysis of  variance  and  F-test  which  revealed a high significant differences among treated and control groups (p&lt;0.05). The results suggest that sensitized spleen cells of guinea pigs has an effect on other recipients and concluded that transfer of sensitized cells were effective in R.equi specific immune response in comparison to the control.}
    }