@article{MAKHILLJAVA2007691267,
    title = {Simultaneous Identification of Tyrosine Hydroxylase and Choline Acetyltransferase Neurons},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {6},
    number = {9},
    pages = {1116-1119},
    year = {2007},
    issn = {1680-5593},
    doi = {javaa.2007.1116.1119},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2007.1116.1119},
    author = {A.O. Ihunwo and},
    keywords = {ChAT,Tyrosine hydroxylase,brain,immunoflurescence,identification,neurons},
    abstract = {Tyrosine Hydroxylase (TH) and Choline Acetyltransferase (ChAT) immunoreactivity were examined in the adult mouse brain by a modified double labelling immunofluorescence  method. Mice  were transcardially perfused with saline followed by fixative (4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4). Frozen brain was cut at 30 m thickness for light microscopy. All staining procedures were conducted under room temperature. Dopaminergic neurons were identified from Cy2-coupled-donkey-anti-rabbit antibody and cholinergic neurons by Cy3-coupleddonkey-anti-goat for ChAT. The cell bodies of both TH- and ChAT- positive neurons with their respective axonal and dendritic processes were easily visible. This method proved reliable for simultaneous demonstration, in the same brain tissue sections, of TH- and ChAT-containing neurons.}
    }