Jesus  Rodriguez-Martinez, Jose A.  Rodriguez-de la Garza, Yolanda  Garza-Garcia, Jose G.  Gaona-Lozano, Leopoldo J.  Rios-Gonzalez, Ileana M.M.  Moreno-Davila, 
    Biohydrogen Production by Anaerobic Biofilms from a Pretreated Mixed Microflora,
    Research Journal of Applied Sciences,
    Volume 5,Issue 6,
    2010,
    Pages 376-382,
    ISSN 1815-932x,
    rjasci.2010.376.382,
    (https://makhillpublications.co/view-article.php?doi=rjasci.2010.376.382)
    Abstract: The present research focused on fermentative production of hydrogen by anaerobic biofilms from a pretreated mixed microflora in a continuous fixed bed-reactor. Microbial mixed culture was thermally (100&deg;C, by 30 min) and acidic (pH 3.0, 24 h) pretreated. Metagenomic analysis of the pretreated microbial cultured was conducted. Microbial cultured community was mainly formed by lactic acid bacillus from <I>Phylum firmicutes</I>. Dairy wastewater (COD 27.73 g L<SUP>-1</SUP>, pH 11.3) at a temperature of 18&plusmn;5&deg;C was fed to a 1.5 L continuous fixed bed-reactor using <I>Opuntia imbricata </I>as substratum. Results obtained showed that at an HRT of 0.4 h the yield hydrogen production was 2.11 mmol H<SUB>2 </SUB>g<SUP>-1</SUP> COD<SUB>consumed</SUB>, mean while an increase in HRT to 0.8 h caused a decrease in yield hydrogen production to 1.31 mmol H<SUB>2 </SUB>g<SUP>-1</SUP> COD<SUB>consumed</SUB>.
    Keywords: dairy wastewater;Opuntia imbricata;metagenomic;hydrogen;Fuel;firmicutes