Bo Kyu  Kang, Myung Han  Park, Jong Man  Kim, Hyoung Joon  Moon, Bong Jo  Kang, Sang Yoon  Han, Se Chang  Park, 
    Phylogenetic Analysis of Viral Hemorrhagic Septicemia Virus (VHSV) Isolated from Olive Flounders (<I>Paralichthys olivaceus</I>),
    Journal of Animal and Veterinary Advances,
    Volume 13,Issue 15,
    2014,
    Pages 952-957,
    ISSN 1680-5593,
    javaa.2014.952.957,
    (https://makhillpublications.co/view-article.php?doi=javaa.2014.952.957)
    Abstract: Viral Hemorrhagic Septicemia Virus (VHSV) is the most important 
  viral pathogens that infects the olive flounders reared in Korean aquaculture. 
  Thirty VHSV isolates were identified in farmed olive flounders from 2007 to 
  2011 by using the Epithelioma Papulosum Cyprini (EPC) cell line. The genetic 
  diversity of the VHSV was evaluated by analyzing the nucleotide sequences of 
  the partial Glycoprotein (<I>G</I>) and Nucleocapsid protein (<I>N</I>) genes 
  of the 30 isolates. Researchers found that all the isolates were closely related 
  to the Japanese and North American genotype IVa which is clearly distinct from 
  the 3 European genotypes. The isolates formed a unique subgroup which was genetically 
  separated from the Japanese and North American isolates. The nucleotide sequences 
  of the 30 isolates exhibited very close identity with each other (&gt;98.6% 
  identity). This study shows that the VHSV genotype IVa strains are widely distributed 
  throughout olive flounder farms in Korea.
    Keywords: Korea;pathogens;glycoprotein;nucleotide;VHSV