He  Dongsheng, Chen  Rui-Ai, Tang  Xu, Wang  Wenhao, Liu  Haopeng, Zhang  Xianhao, 
    Construction of the Fusion Protoplast of Swine <I>Escherichia coli</I> with <I>Hemophilus parasuis</I>,
    Journal of Animal and Veterinary Advances,
    Volume 13,Issue 1,
    2014,
    Pages 1-4,
    ISSN 1680-5593,
    javaa.2014.1.4,
    (https://makhillpublications.co/view-article.php?doi=javaa.2014.1.4)
    Abstract: This is the first study to construct successfully a hybrid 
  protoplast of swine <I>Escherichia coli</I> (sw <I>E. coli</I>) E2010 with <I>Hemophilus 
  parasuis</I> (Hps) H2011 strain. By conventional methods two types of bacteria, 
  identified by specific drug resistant and rabbit-anti antibodies had been fused 
  to construct a fusion protoplast named EH1011. EH1011 showed their parental 
  drug resistance and agglutination identity by rabbit anti-E2010 serum and the 
  rabbit anti-H2011 serum. The genetic characteristics of the fusion strains are 
  stable within 15 generations through the identification of colony morphology, 
  germ staining characteristics, biochemical characteristics and direct agglutination 
  test. This fusion protoplast of swine <I>E. coli</I> and Hps has laid a foundation 
  for the development of a new type of swine bivalent attenuated vaccine to control 
  swine <I>Escherichia coli</I> and <I>Hemophilus parasuis</I>.
    Keywords: Swine Escherichia coli;Hemophilus parasuis;protoplast fusion;vaccine;genetic