Dongying Yang, A Novel PCR-RFLP (Xba I) Polymorphism Analysis of the Bovine Calpastatin (CAST) Gene in Chinese Luxi Cattle, Journal of Animal and Veterinary Advances, Volume 12,Issue 6, 2013, Pages 788-790, ISSN 1680-5593, javaa.2013.788.790, (https://makhillpublications.co/view-article.php?doi=javaa.2013.788.790) Abstract: Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases-μ-calpain and m-calpain, found in mammalian tissues. In the coding region of the bovine CAST gene (CAST) the new nucleotide sequence polymorphism was found being a substitution G→C at position 962 nt (consensus sequence-GenBank AF281256). Consensus of bovine CAST sequence with that of human revealed that G→C substitution was located at position 1460 nt of exon 12. Computer analysis of the mutation showed the Ser→Thr substitution for CAST protein. The frequency of alleles C and G was 0.41 and 0.59, respectively. The message from these results is that CAST gene may probably be considered as a candidate marker for beef quality. Keywords: Genetic polymorphisms;CAST gene;haplotype;RFLP;cattle