S.  Ocak, E.  Emsen, Sinan  Ogun, 
    Ovulation Rate and Embryo Yield of Dorper Sheep in Non-Breeding
Season under Different Superovulatory Protocols,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 6,
    2013,
    Pages 726-730,
    ISSN 1680-5593,
    javaa.2013.726.730,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.726.730)
    Abstract: The response to superovulatory treatment and the embryo yield 
  of Dorper ewes in a non-breeding season were evaluated. Average 65 kg, weight, 
  3 years old donor ewes were inserted CIDR for 12 days and divided into four 
  superovulation treatment groups; Exp. I: 12 mL Folltropin+200 I.U. eCG (n = 
  25); Exp. II: 10 mL Folltropin+200 I.U. eCG (n = 20); Exp. III: 10 mL Folltropin+600 
  I.U. eCG (n = 25) and Exp. IV: 10 mL Folltropin+750 I.U. eCG (n = 10) with single 
  shot of FSH at CIDR removal. Donors were superovulated using FSH-p (total of 
  20 mg) (Folltropin-V; Vetrepharm, Canada) applied in six decreasing doses of 
  3, 2.5, 2, 2, 1.5, 1 mL i.m. starting 60 h before CIDR withdrawal. Donors underwent 
  intrauterine AI with fresh diluted semen (a minimum of 50x10<SUP>6</SUP> motile 
  sperm/each uterine horn) 40 h after CIDR removal and embryos collected 6 days 
  after insemination. The CL responses were found to be similar in all treatment 
  groups (Exp. I: 11.7&plusmn;1.18; II: 9.7&plusmn;1.31; III: 8.4&plusmn;1.76 
  and IV: 9.9&plusmn;1.63). Superovulation response (CL&gt;3) was found relatively 
  but not significantly higher in lower dose of eCG treated Exp. I: 92% and II: 
  91%, respectively compared to III: 76% and IV: 75%. Transferable embryo numbers 
  for a fresh MOET program were found slightly higher (P: 0.333) in donors in 
  Exp. III with 7.8 &plusmn;1.2 than those recorded for donors in Exp. I (4.7&plusmn;1.12), 
  II (6.3&plusmn;1.24) and IV (6.3&plusmn;1.7). The significant difference (p&lt;0.05) 
  was observed in embryo recovery rates; Exp. II and III: 63%, Exp. I: 38% and 
  IV: 43%. Stages of embryonic development were also affected by the different 
  hormonal treatments used in the study Exp. I and IV giving the best results 
  for a fresh MOET program. In conclusion, it was found that Dorper ewes treated 
  with different superovulation protocols in out of season (May) <I>in vivo</I> 
  fresh embryo MOET program yielded 9 embryos and the recovery rate was markedly 
  higher than those of earlier findings. Study also showed that a single FSH injection 
  can be used in place of decreasing dose of multiple FSH injections with similar 
  results.
    Keywords: Embryo transfer;dorper sheep;embryo recovery rate;superovulation;non-breeding season