Duygu  Udum, Cem  Sen, Meltem  Tanriverdi, Hakan  Ustuner, 
    Effects of Peripheral Ghrelin Treatment and the Associations
Between Leptin and Ghrelin in Growing Ram Lambs,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 6,
    2013,
    Pages 694-698,
    ISSN 1680-5593,
    javaa.2013.694.698,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.694.698)
    Abstract: Leptin an adipocytes-derived hormone is an important regulator 
  of bodyweight and energy metabolism. Ghrelin another hormone, influences energy 
  and could directly influence the deposition of fat. In this study, researchers 
  aimed to investigate the effect of long term ghrelin treatment the relationships 
  between leptin and ghrelin and leptin per carcass measurements in Awassi ram 
  lambs. The lambs were randomly assigned to the following 4 groups with 4 animals 
  per group according to both the frequency of food administration and ghrelin 
  treatment: in group I, animals were fed <I>ad libitum</I> in the group II, animals 
  were fed <I>ad libitum</I> and intravenously injected with the ghrelin twice 
  a week in the group III, animals were fed once a day and in the group IV, animals 
  were fed twice a day. Blood samples were collected 30 min before feeding and 
  60 min after feeding to be analyzed for plasma ghrelin and leptin hormone levels. 
  The study was completed using the slaughter weight (43 kg) which was determined 
  when the Awassi lambs in each group were slaughtered after 12 h of fasting. 
  These results suggest that in ruminant species, ghrelin levels are affected 
  by long-term programmed meal feeding; however, the relationships between leptin 
  and carcass weight and MLD measurements showed no significant differences.
    Keywords: Leptin;ghrelin;lambs;MLD area;blood