Qiumei  Shi, Guangping  Gao, Guisheng Gao  , Yanying Zhang  , Donglin Zhang  , Hong Yan Ding  , 
    Detections of Genes Associated with LEE and HPI Pathogenicity Island Which are Comes from Pathogenic <I>E. coli</I> of Minks,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 5,
    2013,
    Pages 655-659,
    ISSN 1680-5593,
    javaa.2013.655.659,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.655.659)
    Abstract: To explore the pathogenesis of causeing diarrhea in mink by 
  <I>E. coli</I>, researchers used PCR to detect the pathogenicity island genes 
  LEE (<I>Ler</I>, <I>eaeA</I>) and HPI (<I>irp</I>, <I>fyuA</I>) of three <I>E. 
  coli</I> whose isolation and identification of serotypes are O38, O78 and O29. 
  Researchers designed four pairs of specific primers according to its gene sequence. 
  The genomic DNA as the template for amplification. Results of the three <I>E. 
  coli</I> are that <I>irp2</I> gene and <I>fyuA</I> gene of HPI were success 
  fully amplified but the LEE (<I>Ler</I>, <I>eaeA</I>) genes were not amplified. 
  Sequence of HPI (<I>irp2</I>, <I>fyuA</I>) showed that the homology about irp2 
  of the three <I>E. coli</I> is between 98.5 and 99.3%. The homology with reference 
  <I>E. coli</I>&#146;s <I>irp2</I> 
  gene is between 98.5 and 99.3%.The homology about fyuA of the three <I> E. coli</I> 
  is between 96.7 and 97.4%. The homology with reference <I>E. coli</I>&#146;s 
  <I>fyuA</I> gene is between 95.8 and 99.4%.
    Keywords: Escherichia coli;virulence gene;LEE and HPI gene;PCR;gene sequence