Leyla  Uslu, Yasar  Durmaz, Oya  Isik, Yasemin  Mutlu, Kemal  Koc, Burcu  Ak, 
    Nitrogen Limitation Increases Lipid Content of <I>Chlorella vulgaris</I> at Photobioreactor System,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 1,
    2013,
    Pages 52-57,
    ISSN 1680-5593,
    javaa.2013.52.57,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.52.57)
    Abstract: This study aimed to produce <I>C. vulgaris</I> which is easy 
  to culture with limited cost and whose outside culture proved practical by increasing 
  its lipid content. <I>Chlorella vulgaris</I> was cultured at photo bioreactor 
  in non-nitrogen nutritive medium with different flow rates (0.3, 0.5 and 0.6 
  m sec<SUP>-1</SUP>) and inoculataion densities of 20 and 50%. The highest lipid 
  content in the study was obtained from the culture with a biomass rate of 38.16&plusmn;0.8% 
  and 0.409&plusmn;0.01 g L<SUP>-1</SUP>, inoculation density of 50% and a flow 
  rate of 0.6 m sec<SUP>-1</SUP>. The lowest lipid content was found as 21.34&plusmn;0.5% 
  in the culture with an inoculation density of 50% and a flow rate of 0.3 m sec<SUP>-1</SUP>. 
  The highest protein content was found as 21.73&plusmn;0.1% in the culture with 
  inoculation density of 50% and a flow rate of 0.5 m sec<SUP>-1</SUP>. The lowest 
  biomass content found as 0.035&plusmn;0.007 g L<SUP>-1</SUP> in the culture 
  with an inoculation density of 20% and a flow rate of 0.3 m sec<SUP>-1</SUP>. 
  The lipid content in that culture was found as 29.69&plusmn;1% and the protein 
  content as 14&plusmn;0.5%. These results suggest that N limitation increases 
  <I>C. vulgaris</I> lipid content.
    Keywords: N limitation;C. vulgaris;lipid;biomass;photobioreactor system;Turkey