Shuangfen Yue, Saifei Li, Huaan Wen, Li Fan,
Isolation, Identification and Antioxidant Properties of Xylaria escharoidea Associated with Termite Nest,
Journal of Animal and Veterinary Advances,
Volume 12,Issue 3,
2013,
Pages 330-336,
ISSN 1680-5593,
javaa.2013.330.336,
(https://makhillpublications.co/view-article.php?doi=javaa.2013.330.336)
Abstract: The strain of Xylaria Y-4 was isolated from an active termite nest and identified as Xylaria escharoidea based on ITS1-5.8S-ITS2 sequences. Intracellular Polysaccharide (IPS) and Extracellular Polysaccharide (EPS) were extracted from Xylaria escharoidea (strain Y-4) in submerged culture. Antioxidant potency was investigated by assays of various established antioxidant in vitro systems such as hydroxyl (AOH) and DPPH radical scavenging ability, reducing power and ferrous ion chelating ability. Results revealed that the antioxidant activities of IPS extracts were generally more potent than EPS extracts in in vitro assays. IPS exhibited stronger scavenging activity towards DPPH radicals with EC50 of 0.75 mg mL-1.
Keywords: Free radicals;polysaccharide;reducing power;IPS;in vitro