Caoting Xing  Yiqiao, Yang  Yanning, Wang  Xiaoxiong, 
    Study of MMP-2 and FAK Expression in HSV-1 Infected Human Corneal Epithelial Cells,
    Journal of Animal and Veterinary Advances,
    Volume 12,Issue 22,
    2013,
    Pages 1632-1637,
    ISSN 1680-5593,
    javaa.2013.1632.1637,
    (https://makhillpublications.co/view-article.php?doi=javaa.2013.1632.1637)
    Abstract: To investigate Matrix Metallo Proteinases-2 (MMP-2) and Focal Adhesion Kinase (FAK) expression in Herpes Simplex Virus-1 (HSV-1) infected human corneal epithelial cells. Human corneal epithelial cells were infected with HSV-1 <I>in vitro</I> to establish cell model of viral infection. The expression of MMP-2 and FAK were detected by RT-PCR, Western blot, Immunohistochemical and Immumofluorescence Method at 2, 20 and 40 h post-infection. Results at 2, 20 and 20 h post-infection, the mRNA of MMP-2 and FAK expression were significantly increased compared with uninfected cells (p&lt;0.01, p&lt;0.05). At 2 h post-infection, the expression of MMP-2, FAK and Phosphorylation FAK (P-FAK) protein were not significantly different with uninfected cells (p&gt;0.05). At 20 and 40 h post-infection, the expression of MMP-2, FAK and P-FAK protein were significantly increased compared with uninfected cells (p&lt;0.05). At the initial stage of HSV-1 infected, P-FAK plays an important role in the process of virus invading and MMP-2 activation.
    Keywords: MMP-2;FAK;HSV-1 keratitis;virus;cells